Part:BBa_K2680263

3G AraC-ssrA

AraC gene from the L-arabinose operon with an added ssra tag to allow for quicker endogenous degradation in e.coli. Represses pBAD promoter in the absence of arabinose. Gene flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

Compare to the coding sequence for AraC, BBa_K1088005. From this page:

"This sequence is a copy of the sequence 70,387 -> 71,265 from E. coli K-12 MG1655 which is the coding sequence of the pBAD repressor araC. From ECOCYC: "The "arabinose regulator," AraC, is a transcription factor that regulates transcription of several genes and operons involved in arabinose catabolism and transport. It coregulates with another transcriptional regulator, CRP; both are transcription factors involved in l-arabinose degradation. These regulators bind cooperatively to activate transcription of five operons related to transport, catabolism, and autoregulation of l-arabinose. Transcription of these operons is induced when E. coli is grown in the absence of glucose and when the physiological inducer, l-arabinose, binds to the AraC regulator. In the absence of glucose, cellular cyclic AMP levels are high and cyclic AMP forms a dimeric complex with CRP to coregulate with AraC." For more info go to [http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10054 Ecocyc's page]"¹

References

¹Kj, A. (2013, August 20). Part:BBa_K1088005. Retrieved October 16, 2018, from https://parts.igem.org/Part:BBa_K1088005

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 47
  Illegal BsaI.rc site found at 978